Coding

Part:BBa_K3498030

Designed by: Raúl Acosta Murillo   Group: iGEM20_FCB-UANL   (2020-09-25)


Foam stabilization proteins Ranaspumin-3, Ranaspumin-4, Ranaspumin-5

This piece is intended to generate three foam stabilizing proteins which are ranaspumina-3, ranaspumina-4, ranaspumina-5. This piece contains an adjustable vanillinic acid sensitive promoter, three binding ribosome regions, three coding sites and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 281
    Illegal PstI site found at 706
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal PstI site found at 281
    Illegal PstI site found at 706
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1141
    Illegal BamHI site found at 4
    Illegal BamHI site found at 42
    Illegal BamHI site found at 761
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 281
    Illegal PstI site found at 706
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 281
    Illegal PstI site found at 706
    Illegal AgeI site found at 102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1780


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Categories
Parameters
None